By John H. Rothman, Andrew Singson
An up-to-date variation of the classic Methods in phone Biology quantity forty eight, this book emphasizes different equipment and applied sciences had to examine C. elegans, either as an built-in organism and as a version process for learn inquiries in telephone, developmental, and molecular biology, in addition to in genetics and pharmacology. by means of directing its audience to tried-and-true and state of the art recipes for research, this complete assortment is meant to lead investigators of C. elegans for future years. *Diverse, up to date suggestions covered will be invaluable to the broadening neighborhood of C. elegans researchers for future years* Chapters written by way of leaders within the box* attempted and real equipment carry busy researchers a one-stop compendium of crucial protocols
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Extra resources for Caenorhabditis elegans: Molecular Genetics and Development
This group includes duplications and transgenic arrays. It is useful to have marked balancers expressing a recognizable phenotype (Tables I–III). , 2007). Equally valuable for strain maintenance are dominant markers. An excellent example of a dominant marker is an insertion of a transgene that express GFP, such as myo-2::GFP, which expresses GFP in the pharynx. Popular examples of balancers that have insertions of myo-2::GFP are the inversion, mIn1 and the translocations, nT1 and hT2 (Table I).
Methods Cell Biol. 48, 81–96. Williams, B. , and Waterston, R. H. (1992). A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites. Genetics 131, 609–624. , Collins, A. , and Day, I. N. (2001). An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res. 29, E88–E98. CHAPTER 2 Specialized Chromosomes and Their Uses in Caenorhabditis elegans Martin R. Jones, Zoe Lohn and Ann M. Rose Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada Abstract I.
Methods for Creating Transgenic Animals E. Use of Transgenic Arrays as Balancers Acknowledgments References Glossary Abstract Research on Caenorhabditis elegans involves the use of a wide range of genetic and molecular tools consisting of chromosomal material captured and modified for specific purposes. These ‘‘specialized chromosomes’’ come in many forms ranging from relatively simple gene deletions to complex rearrangements involving endogenous METHODS IN CELL BIOLOGY, VOL 106 Copyright 2011, Elsevier Inc.
Caenorhabditis elegans: Molecular Genetics and Development by John H. Rothman, Andrew Singson