By Lalitha Prajna
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Additional info for Aravind's atlas of fungal corneal ulcers: clinical features and laboratory identification methods
Inoculation of fungal colony B. Dematiaceous fungus grown well after 5 days C. Well grown hyaline fungus ready to be observed for the morphological form of the fungal structures will be in the same plane, making examination easier. This detail is especially important for photography. In addition, an excess of liquid will prevent proper sealing of the cover slip in any attempt to make a permanent mount of the preparation. More enduring mounts can be produced by placing a small drop of lactophenol cotton blue on the cover slip bearing the fungal growth and covering it with a second cover slip of smaller size.
Mix and cover with glass cover slip. 4. Allow to sit at room temperature until material is clear (warming slide will hasten process). 5. Examine using a fluorescence microscope with 10X and 40X to 50X objective or 100X oil. Interpretation: Fungi stain bright green or blue-white, depending on the filter. 5 Recommendations for Isolation of Fungi from Ocular Specimens RECOMMENDED MEDIA FOR FUNGI ISOLATION Clinical specimens are processed promptly and plated to isolation media as a means to recover fungi that may be causing disease.
Media must be carefully selected based on specimen type and fungal suspected agents. Media is dispensed into bottles or 100 mm Petri dishes. Petri plates offer the advantage of a large surface area for isolation and dilution of inhibitory substances in the specimens, but must be poured thick with at least 25 ml of medium to resist dehydration during incubation. Because plates are vented, they are more likely to become contaminated during incubation. Each Petri plate must be labeled on the bottom, and the lid must be taped at two points to prevent accidental opening of the plate.
Aravind's atlas of fungal corneal ulcers: clinical features and laboratory identification methods by Lalitha Prajna