Get Animal Cell Culture PDF

By Mohamed Al-Rubeai (eds.)

ISBN-10: 3319103199

ISBN-13: 9783319103198

ISBN-10: 3319103202

ISBN-13: 9783319103204

​Animal cells are the popular “cell factories” for the creation of advanced molecules and antibodies to be used as prophylactics, therapeutics or diagnostics. Animal cells are required for the proper post-translational processing (including glycosylation) of biopharmaceutical protein items. they're used for the construction of viral vectors for gene treatment. significant goals for this treatment contain melanoma, HIV, arthritis, cardiovascular and CNS ailments and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological stories. This booklet is designed to function a finished evaluation of animal cellphone tradition, overlaying the present prestige of either study and functions. For the scholar or R&D scientist or new researcher the protocols are important to the functionality of cellphone tradition paintings, but a wide knowing is vital for translation of laboratory findings into the commercial creation. in the vast scope of the publication, every one subject is reviewed authoritatively via specialists within the box to provide state of the art choice of present examine. a tremendous reference quantity on mobilephone tradition study and the way it affects on construction of biopharmaceutical proteins world wide, the booklet is vital analyzing for everybody operating in telephone tradition and is a advised quantity for all biotechnology libraries.

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Cell 56:619–630 Thomason L, Court DL, Bubunenko M, Costantino N, Wilson H, Datta S, Oppenheim A (2007) Recombineering: genetic engineering in bacteria using homologous recombination. Curr Protoc Mol Biol. 16 Tichy ED, Pillai R, Deng L, Liang L, Tischfield J, Schwemberger SJ, Babcock GF, Stambrook PJ (2010) Mouse embryonic stem cells, but not somatic cells, predominantly use homologous recombination to repair double-strand DNA breaks. Stem Cells Dev 19:1699–1711 1 Cell Line Development 25 Turan S, Galla M, Ernst E, Qiao J, Voelkel C, Schiedlmeier B, Zehe C, Bode J (2011) Recombinasemediated cassette exchange (RMCE): traditional concepts and current challenges.

2 Vector Elements Vectors for transient transfection require elements that are necessary or support recombinant protein production. Necessary elements are the promoter, the recombinant protein’s coding sequence and a signal for transcription stop and polyadenylation. g. by stabilizing recombinant transcripts. 34 V. Ja¨ger et al. Antibody gene sequences were evolved for maximum expression. The coding sequence and untranslated transcript regions of other genes often benefits from optimization (Fath et al.

J Biotechnol 124:457–468 Cost GJ, Freyvert Y, Vafiadis A, Santiago Y, Miller JC, Rebar E, Collingwood TN, Snowden A, Gregory PD (2010) BAK and BAX deletion using zinc-finger nucleases yields apoptosisresistant CHO cells. Biotechnol Bioeng 105:330–340 Cradick TJ, Fine EJ, Antico CJ, Bao G (2013) CRISPR/Cas9 systems targeting beta-globin and CCR5 genes have substantial off-target activity. Nucleic Acids Res 41:9584–9592 Crawford Y, Zhou M, Hu Z, Joly J, Snedecor B, Shen A, Gao A (2013) Fast identification of reliable hosts for targeted cell line development from a limited-genome screening using combined phiC31 integrase and CRE-Lox technologies.

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Animal Cell Culture by Mohamed Al-Rubeai (eds.)

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