By Oscar; Latner, A. L. Bodansky
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Extra info for Advances in Clinical Chemistry, Vol. 19
5 . 4 Values are based on replicate analyses of a singlc specimen or on randomized duplicate estimations on different specimens and refer to within-batch reproducibility unless otherwise stated. 43 AUTOMATIC ENZYME ANALYZERS A particularly impressive feature of the Gilford 3500 automatic enzyme analyzer is the rapid changeover that is possible from one temperature of measurement to another: a new indicated temperature is reached within seconds of changing from a different setting. O5"C. It was not possible to check the accuracy of the temperature indication in this laboratory, but in order to test whether the instrument returned reproducibly to a particular temperature when reset from a different temperature, a series of replicate determinations of lactate dehydrogenase in pooled serum were made: 10 measurements were made at 37"C, the setting was changed to 25°C and 10 further measurements were made, followed by 10 replicates at 37°C and finally 10 at 25°C.
Adjustment of the position of the reagent-addition heads along a notched bar allows the lapse of time to be varied between sample dispensing and further reagent additions, or between these operations and introduction of the reaction mixture into the spectrophotometer cuvette. AUTOMATIC ENZYME ANALYZERS 39 FIG. 17. The Gilford 3500 automatic enzyme analyzer. ) The spectrophotometer is a single-beam diffraction-grating instrument with a wavelength range of 340-700 nni (bandwidth 8 nm), with direct readout of absorbance to 2 A.
12. Histogram of the agreement between duplicate estimations of lactate dehydrogenase activity in serum by a manual spectrophotoinetric method at 25°C (open bars) and by the LKB 8600 reaction-rate analyzer at 35°C (filled bars). Differences between duplicates are expressed as a percentage of the mean value for each pair. 05'C (Al). The 24 readings collected from each of the 24 cuvettes within a 2-minute period are entered into the 16K memory of a Nova 1200 computer. The computer is programmed to search for the appropriate linear portion of the curve of absorbance change with time.
Advances in Clinical Chemistry, Vol. 19 by Oscar; Latner, A. L. Bodansky